BD LSR Fortessa SORP – How to Use Guide 3 Section 2: Switch from acquisition in plate to acquisition in tubes The standard configuration of the cytometer is to acquire samples in 96-well plates using the High Throughput Sampler (HTS). If you need to switch the configuration to acquire your samples in FACS tubes, please follow these steps:
Flow Cytometry Service BD LSR Fortessa X20 – How to Use Guide 5 13 Install the unstained control sample in the cytometer and set the speed to LOW. Start acquisition in the Acquisition Dashboard. 14 In the Cytometer panel, in the Parameters tab, adjust the voltages of FSC and SSC in order to roughly place your cells of interest at the center of the dotplot.
When you are finished and if you are not the last booked user for the day, log out of your centre folder by selecting File > Log Out. The screen should look like this ready for the next user. NB PLEASE DO NOT LOG OUT OF THE FACS DIVA SOFTWARE AS THIS MAY CAUSE CONNECTIVITY PROBLEMS FOR THE NEXT USER. 14. The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, Technical and application support personnel are available for help in workflow, the optional BD™ High Throughput Sampler (HTS) provides rapid, fu BD LSR Fortessa Flow Cytometry Analyzer equipped with HTS (High Throughput Sampler) BD FACSDiva HTS quick reference guide · HTS quick reference AMREP FLOW – LSR-Fortessa Users SOP. V1.2 Sept 2013 of the HTS, you will have to wait for the HTS to go through its prime. 3. Check the fluidics. When logged in, click the BD FACS DIVA software icon.
With up to four different laser configurations and 14 parameters, the cytometer is fully optimized to work with BD Horizon Brilliant ™ dyes, so you can focus on what matters most—your research. SOP for HTS Getting started Switch the cytometer and the computer on. The HTS initialises when cytometer is switched on. Login to DIVA software. Before proceeding with the HTS, clean the instrument according to standard AMREPFLOW SOPs: • Check fluidics: empty waste and fill sheath.
SOP for HTS Getting started Switch the cytometer and the computer on. The HTS initialises when cytometer is switched on. Login to DIVA software. Before proceeding with the HTS, clean the instrument according to standard AMREPFLOW SOPs: • Check fluidics: empty waste and fill sheath.
Revision. Date. Change The BD LSR Fortessa™ X-20 cell analyzer delivers high-performance HTS platereader Quickstart guide · BD FACSFlow Supply System User Guide · BD A full list of all the manuals, guides and webinars are located on the BD Lasers and filters configuration available in our BD Fortessa II for Class II work.
Breakthrough with the BD FACSCelesta™ Cell Analyzer. Unleash the power of the core on your own benchtop. Leveraging high performance in a smaller footprint, the BD FACSCelesta gives you the independence to go beyond and drive deeper scientific discoveries.
HTS throughput Acquisition: Less than 15 minutes per microtiter plate in high throughput mode using a 2-second Stanford LSRFortessa User Guide 100710 Starting up 1. Turn on the computer. Wait for the computer to boot up. 2. Log in to the computer (facsuser, password =BDIS) and open BD FACSDiva software.
Optimizing Setup of the LSR2 system . LSR2 Optimization Summary . 9. Tracking settings with Rainbow Beads . 10.
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HTS Throughput Acquisition: Less than 15 minutes per microtiter plate in high-throughput mode using a 2-second http://stratedigm.com offers affordable flow cytometer as an alternative to the BD LSR II & BD Fortessa.Call( 408) 512-3901 to learn more about our flow cyto BD LSRIIs and BD LSR-Fortessa have performance, capabilities, and flexibility never seen before in a benchtop analyzer. The BD LSRIIs and BD LSR-Fortessa can be configured with up to five fixed alignment lasers and the ability to detect up to 18 colors, utilizing a revolutionary optical design. We have three BD LSR Fortessa SORP (Special Order Research Product) instruments in our facility. Fortessa 1 and 2 are equipped with 5 lasers (405nm, 448nm, 488nm, 561nm and 633nm) detecting up to 18 colors simultaneously plus forward and side scatter signals. These analyzers are located in U1.44 and therefore only BSL-1 samples can be acquired.
Power:__50_mW. 4 juni 2016 — Starta hög genomströmning Sampler (HTS) i kombination med LSR FORTESSA cytometer, BD Biosciences, France, 649225B4. TECNAI G2
22 jan. 2019 — Det första steget i strategin Usenets baseras på framåt och side scatter, vilket gör och flödescytometri med robotarmar och HTS-plattformen, Vi) snabb härdning av data med LSR Fortessa, BD Biosciences, Flow cytometer (4 lasers-blue, red, violet and yellow) PDF DOI DOWNLOAD MATERIALS LIST
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The BD™ High Throughput Sampler (HTS) option is available to increase your lab productivity by acquiring samples from a 96- or 384-well microtiter plate. The HTS can be front or side mounted on the BD LSRFortessa X-20. HTS Throughput Acquisition: Less than 15 minutes per microtiter plate in high-throughput mode using a 2-second
Refill fluids and empty waste, if needed, and then select Cytometer > Long Clean . When the long clean is complete, run a fluidics shutdown and turn off the system. BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1. Turn on the instrument: a. LSR Fortessa - red switch on the wall behind the monitors (Fig. A1). b.
to using the BD™ High Throughput Sampler (HTS; Plate Loader) on the DHVI Flow Facility L01 and L02 bench-top analyzers. 2.0 Scope and Application This SOP applies specifically to the BD LSRII (L01/L02) instruments in the MSRBII Building, Hallway 2045, Bay 5 and 6. This procedure applies to Flow
Before proceeding with the HTS, clean the instrument according to standard AMREPFLOW SOPs: • Check fluidics: empty waste and fill sheath. A while back, we were recommended away from routine PRIME functions for this very reason (BD service recommendation).
With superior optics and fluidics for maximized sensitivity and resolution, the BD LSRFortessa™ helps you analyze your sample with ease. It processes 96 samples under 15 minutes with less than 0.5% sample carryover. Cited in over 4,500 publications, the BD LSRFortessa is your next step to discovery. This 4.5 days course teaches the operation of the BD LSRFortessa, BD LSRFortessa X-20 and BD FACSCelesta. Additionally, the challenges of successful multi-parameter analysis in terms of antibody-fluorochrome selection, instrument setup including High Throughput Sampler (HTS) & Quality Control (BD™ 2018-12-11 · BD_LSR-Fortessa_18-COLOR_20181211 Cytometer: LSRFortessa Cytometer Name: LSRFortessa Serial Number: H64780000027 Input Device: HTS Window Extension: 10.00 User: Administrator Institution: N/A Software: BD FACSDiva 8.0.1 Date: 12/11/2018 2:59:48 PM Blue Laser (488nm) FSC Bringing boundless potential to multicolor flow cytometry, the BD LSRFortessa X-20 cell analyzer gives you the power to expand and customize your science. Built with innovative optical efficiency and fluidics optimized for sensitivity, this system is backed by BD expert training, service and support—so there is no limit to your research potential.